Characterization and optimization of enzyme production by nonconventional fungi

Master´s thesis: Fungal Biotechnology

26.01.2026, Abschlussarbeiten, Bachelor- und Masterarbeiten

The Werner Siemens Chair of Synthetic Biotechnology at the Department of Chemistry at the Technical University of Munich in Garching focuses on the isolation, characterization, and process-oriented optimization of enzymes and microorganisms for the production of industrial platform chemicals. A key research area is the fermentation of filamentous fungi with the aim of producing extracellular proteins, natural products and pigments for a variety of industrial applications.

🧬 Topic:
Characterization and optimization of enzyme production by nonconventional fungi


⚛️ Your responsibilities:

• Cultivation and characterization of unconventional filamentous fungal strains

• Performance of various enzyme assays, particularly for the detection of lipase activity

• Optimization of enzyme production in a filamentous fungal strain in liquid culture using statistical experimental design

• DNA isolation, whole genome sequencing (PacBio HiFi sequencing) and analysis of genomic data

• Generation of proteomic data (LC-MS/MS) for analysis of enzyme production

• Upscaling of the optimized fermentation process (up to 50 L)


✅ Your qualifications:

We are looking for a self-motivated, team-oriented, and well-structured individual with experience in biochemistry,
biotechnology or microbiology. Previous experience in cultivation of microorganisms and with molecular biology
methods are advantageous.


☑️ A research internship can be completed before starting the master's thesis.
The position is available from mid-March.


We look forward to receiving your application documents.

ℹ️ Contact information:
Annika Krimmel, M.Sc.
annika.krimmel@tum.de
Werner Siemens-Lehrstuhl für Synthetische Biotechnologie (WSSB)
Department Chemie, Technische Universität München (TUM)
Lichtenbergstraße 4, 85748, Garching bei München

Kontakt: annika.krimmel@tum.de

Mehr Information

https://www.ch.nat.tum.de/wssb/startseite/